Species-specific ribosome stalling

Sohmen, D., Chiba, S., Shimokawa-Chiba, N., Innis, C.A., Berninghausen, O., Beckmann, R., Ito, K., Wilson, D.N. (2015). Structure of the Bacillus subtilis 70S ribosome reveals the basis for species-specific stalling. Nat Commun 6, 6941.

PDB entries: 3J9W

Collaboration with the Wilson, Ito and Beckmann groups to reveal the mechanism by which nascent MifM peptide causes ribosomes from the Gram-positive bacterium Bacillus subtilis to undergo translational arrest. Comparison with ribosomes from Gram-negative bacteria such as Escherichia coli reveals that a single amino acid within ribosomal protein uL22 dictates species-specific stalling.